Single-cell profiling of AKI in a murine model reveals novel transcriptional signatures, profibrotic phenotype, and epithelial-to-stromal crosstalk
V Rudman-Melnick, M Adam, A Potter… - Journal of the …, 2020 - journals.lww.com
Background Current management of AKI, a potentially fatal disorder that can also initiate or
exacerbate CKD, is merely supportive. Therefore, deeper understanding of the molecular
pathways perturbed in AKI is needed to identify targets with potential to lead to improved
treatment. Methods We performed single-cell RNA sequencing (scRNA-seq) with the
clinically relevant unilateral ischemia-reperfusion murine model of AKI at days 1, 2, 4, 7, 11,
and 14 after AKI onset. Using real-time quantitative PCR, immunofluorescence, Western …
exacerbate CKD, is merely supportive. Therefore, deeper understanding of the molecular
pathways perturbed in AKI is needed to identify targets with potential to lead to improved
treatment. Methods We performed single-cell RNA sequencing (scRNA-seq) with the
clinically relevant unilateral ischemia-reperfusion murine model of AKI at days 1, 2, 4, 7, 11,
and 14 after AKI onset. Using real-time quantitative PCR, immunofluorescence, Western …
